cd87 apc  (Miltenyi Biotec)

Journal: Cell Reports Methods

Article Title: MPA PASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays

doi: 10.1016/j.crmeth.2021.100136

Figure Lengend Snippet: Detection antibodies used

Article Snippet: CD87 , 0.5 μg , APC , recombinant human IgG1 , REA892 , Miltenyi Biotec , 130-114-851.

Techniques: Labeling, Recombinant

Journal: Cell Reports Methods

Article Title: MPA PASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays

doi: 10.1016/j.crmeth.2021.100136

Figure Lengend Snippet: Detection antibodies used

Article Snippet: CD87 , Miltenyi Biotec , Cat# 130-114-851; RRID: AB_2726804.

Techniques: Labeling, Recombinant

Journal: Cell Reports Methods

Article Title: MPA PASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays

doi: 10.1016/j.crmeth.2021.100136

Figure Lengend Snippet:

Article Snippet: CD87 , Miltenyi Biotec , Cat# 130-114-851; RRID: AB_2726804.

Techniques: Clinical Proteomics, Recombinant, Saline, Modification, Software

Journal: Cell Reports Medicine

Article Title: A GPC2 antibody-drug conjugate is efficacious against neuroblastoma and small-cell lung cancer via binding a conformational epitope

doi: 10.1016/j.xcrm.2021.100344

Figure Lengend Snippet:

Article Snippet: CD87 Antibody, Anti-Human (APC, Clone VIM5) , Miltenyi Biotec , Cat # 130-099-456; RRID: AB_2659423.

Techniques: Control, Western Blot, Virus, Cell Culture, Recombinant, Expressing, Transfection, Cell Viability Assay, Caspase-Glo Assay, Luciferase, Mutagenesis, Antibody Labeling, Sonication, Chromatin Immunoprecipitation, SYBR Green Assay, Fluorescence, Quantitation Assay, Conjugation Assay, Gene Expression, Plasmid Preparation, shRNA, Software

Journal: Journal of Intensive Care

Article Title: Neutrophils are a main source of circulating suPAR predicting outcome in critical illness

doi: 10.1186/s40560-019-0381-5

Figure Lengend Snippet: Expression of urokinase receptor (uPAR) by circulating immune cells. Immune cells were isolated from peripheral blood of 27 healthy volunteers (HC), 48 standard care patients with bacterial infections (SC) as well as 87 intensive care patients (ICU) and analyzed using multicolor flow cytometry. a Neutrophils were characterized as CD15 + cells, monocytes by expression of CD14 + and/or CD16 + , B cells as CD19 + , and T cells as CD3 + CD56 − cells. b The expression of uPAR (CD87) by immune cell subsets was assessed using multicolor flow cytometry. Representative histograms are shown, the white histogram shows isotype control staining. c Quantification of uPAR expression on immune cell subsets, based on % of positive cells by flow cytometry. One-way ANOVA and Kruskal-Wallis test (non-parametric) with Dunn’s multiple comparison test were done to compare groups. Significant differences are indicated by * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: All cells were stained with a monoclonal APC-conjugated mice anti-human CD87 antibody (eBioscience, San Diego, USA, clone VIM5) or IgG2b isotype as well as with CD15, CD8, and CD16 (all from BD Horizon/Pharmingen, Heidelberg Germany) and CD11b, CD14, CD56, CD3, CD4, and CD19 (all from eBioscience, San Diego, USA) [ ].

Techniques: Expressing, Isolation, Flow Cytometry, Control, Staining, Comparison

Journal: Journal of Intensive Care

Article Title: Neutrophils are a main source of circulating suPAR predicting outcome in critical illness

doi: 10.1186/s40560-019-0381-5

Figure Lengend Snippet: Association between neutrophilic uPAR expression and circulating suPAR in critically ill patients. Flow-cytometric expression of uPAR (CD87) on circulating neutrophils and serum levels of suPAR were analyzed in 27 healthy volunteers, 48 standard care patients with bacterial infections, and 87 intensive care patients at baseline. a Correlation analysis of uPAR expressed on neutrophils and suPAR in peripheral blood in the different cohorts. Correlation coefficient ( r ) and p values are based on Spearman rank correlation test and given in the figure. b uPAR expression by neutrophils and suPAR serum concentrations in septic and non-septic ICU patients. c uPAR expression by neutrophils and suPAR serum concentrations in ICU patients, dependent on the stage of acute kidney injury (AKI) as defined by the Acute Kidney Injury Network (AKIN). Significant differences are indicated by * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: All cells were stained with a monoclonal APC-conjugated mice anti-human CD87 antibody (eBioscience, San Diego, USA, clone VIM5) or IgG2b isotype as well as with CD15, CD8, and CD16 (all from BD Horizon/Pharmingen, Heidelberg Germany) and CD11b, CD14, CD56, CD3, CD4, and CD19 (all from eBioscience, San Diego, USA) [ ].

Techniques: Expressing

Journal: Journal of Intensive Care

Article Title: Neutrophils are a main source of circulating suPAR predicting outcome in critical illness

doi: 10.1186/s40560-019-0381-5

Figure Lengend Snippet: Prognostic relevance of neutrophilic uPAR expression and circulating suPAR levels. Flow-cytometric expression of uPAR (CD87) on circulating neutrophils and serum levels of suPAR were measured at admission to the ICU and 48 h later (day 3). a Neutrophilic uPAR expression and circulating suPAR levels in ICU patients, divided into patients that survived and patients that died at the ICU. b Kaplan-Meier survival curves display the different survival rates of ICU patients, depending on either high or low neutrophilic uPAR (cut-off 75%) or circulating suPAR levels (cut-off 11.95 ng/ml) at ICU admission. c ROC curve analyses comparing the predictive power of suPAR (AUC 0.727) and uPAR (AUC 0.665) at admission for mortality with the established clinical ICU score APACHE II (AUC 0.781). Abbreviations: APACHE Acute Physiology and Chronic Health Evaluation, AUC area under the curve, ROC receiver operating characteristics

Article Snippet: All cells were stained with a monoclonal APC-conjugated mice anti-human CD87 antibody (eBioscience, San Diego, USA, clone VIM5) or IgG2b isotype as well as with CD15, CD8, and CD16 (all from BD Horizon/Pharmingen, Heidelberg Germany) and CD11b, CD14, CD56, CD3, CD4, and CD19 (all from eBioscience, San Diego, USA) [ ].

Techniques: Expressing